https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52054 Wed 27 Sep 2023 15:30:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35372 Wed 08 Jul 2020 10:53:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47290 Tue 30 Apr 2024 08:50:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48671 Tue 28 Mar 2023 10:25:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41985 Tue 16 Aug 2022 15:55:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43133 Tue 13 Sep 2022 15:14:32 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38505 Thu 18 Nov 2021 09:58:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34955 Thu 17 Feb 2022 09:32:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46730 Thu 01 Dec 2022 10:28:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38585 in vitro airway epithelial infection models using high multiplicity of infection (MOI) and lacking genome-wide, time course analyses have obscured the role of epithelial innate anti-viral immunity in asthma and COPD. To address this, we developed a low MOI rhinovirus model of differentiated primary epithelial cells obtained from healthy, asthma and COPD donors. Using genome-wide gene expression following infection, we demonstrated that gene expression patterns are similar across patient groups, but that the kinetics of induction are delayed in cells obtained from asthma and COPD donors. Rhinovirus-induced innate immune responses were defined by interferons (type-I, II, and III), interferon response factors (IRF₁, IRF₃, and IRF₇), TLR signaling and NF-κB and STAT₁ activation. Induced gene expression was evident at 24 h and peaked at 48 h post-infection in cells from healthy subjects. In contrast, in cells from donors with asthma or COPD induction was maximal at or beyond 72–96 h post-infection. Thus, we propose that propensity for viral exacerbations of asthma and COPD relate to delayed (rather than deficient) expression of epithelial cell innate anti-viral immune genes which in turns leads to a delayed and ultimately more inflammatory host immune response.]]> Mon 29 Jan 2024 18:03:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40389 Oxyuranus scutellatus from four localities on the north-east coast of Australia, spanning a distance of 2000 km. The intra-specific variation in taipan venom was considerably less than the inter-specific variation between it and the other Australian elapids to which it was compared. The electrophoretic venom profile of O. scutellatus was visually different to six other genera of Australian elapids, but not to its congener inland taipan O. microlepidotus. There was minimal geographical variation in taipan venom, as the intra-population variation exceeded the inter-population variation for enzymatic activity, procoagulant activity, and the abundance of neurotoxins. The pre-synaptic neurotoxin (taipoxin) was more abundant than the post-synaptic neurotoxins (3FTx), with a median of 11.0% (interquartile range (IQR): 9.7% to 18.3%; range: 6.7% to 23.6%) vs. a median of 3.4% (IQR: 0.4% to 6.7%; range: 0% to 8.1%). Three taipan individuals almost completely lacked post-synaptic neurotoxins, which was not associated with geography and occurred within two populations. We found no evidence of sexual dimorphism in taipan venom. Our study provides a basis for evaluating the significance of intra-specific venom variation within a phylogenetic context by comparing it to the inter-specific and inter-generic variation. The considerable intra-population variation we observed supports the use of several unpooled individuals from each population when making inter-specific comparisons.]]> Mon 11 Jul 2022 11:21:41 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51239 Fri 10 Nov 2023 07:15:55 AEDT ]]>